Lymphocyte proliferative responses in dairy cows supplemented with an immunomodulatory feed additive and administered polyvalent vaccination / [Proliferação de linfócitos em vacas leiteiras suplementadas com um aditivo alimentar imunomodulador e vacinadas com vacina polivalente]

O presente estudo avaliou o efeito da suplementação com OmniGen-AF® na proliferação de linfócitos e títulos de anticorpos após vacinação em bovinos leiteiros. Amostras de sangue periférico foram coletadas de 32 vacas leiteiras para quantificação dos títulos de anticorpos anti-Leptospira, e amostras de sangue periférico de 16 vacas leiteiras foram também coletadas para avaliação da proliferação de linfócitos. Observou-se que a suplementação com OmniGen-AF® aumentou a proliferação basal de linfócitos (sem estímulos) 21 dias após a vacinação (P=0,03), apesar de reduzir a proliferação de linfócitos B quando estimulada com Leptospira borgpetersenii serovar Hardjo inativada pelo calor (P=0,03). Ademais, nenhum efeito da suplementação sobre a proliferação de linfócitos no momento imediatamente anterior à vacinação e nos títulos de anticorpos anti-Leptospira foi encontrado. Além disso, a proliferação de linfócitos estimulada com lipopolissacarídeos foi maior em vacas multíparas que em primíparas 21 dias após a vacinação (P=0,03). Desse modo, o presente estudo demonstra que a suplementação com OmniGen-AF® não afetou de forma robusta a proliferação de linfócitos e os títulos de anticorpos anti-Leptospira após vacinação em vacas leiteiras sadias.(AU)

[Depth of the curve of Spee in relation to FMA, FH, ANB and dental class]. / Profondità della curva di Spee in rapporto a FMA, FH, ANB e classe dentale.

The Authors have statistically evaluated (by student's t test) a series of 151 patients to evaluate the relation between the depth of Spee's curve and some skeletal cephalometric (FMA, FH and ANB) and occlusal (dental Class) parameters. The test has showed that there are not significant variations of Spee's curve depth in relation with vertical skeletal parameters (FMA and FH); on the contrary, it has showed significant variations as regards horizontal (ANB) and occlusal (dental Class) parameters. Particularly, the average value of Spee's curve of skeletal and dental III Classes has turned out to be systematically lower than that of other Classes.

Rowell's syndrome. Report of a case.

We describe a patient with discoid lupus erythematosus who developed annular lesions of the thigh and chilblainlike lesions of the fingers matching those described in the original reports of Rowell's syndrome. The patient also had circulating anti-Ro(SS-A) antibodies whose similarity to the anti-Sj-T antibodies found in the original Rowell's syndrome cases has been recently claimed. A review of the literature suggests that most of the cases of Rowell's syndrome described thus far in fact may be cases of coincidental association of lupus erythematosus and erythema multiforme.

Growth and sport.

In order to investigate sport's influence on growth during puberty, we examined 398 boys 10-16 yr old. 192 of these subjects had been playing football as a competitive sport, and 206 were considered as controls, having never practised sports regularly. Both groups were divided into prepubertal (with testicular volume less than or equal to 2.5 cc) and pubertal, with the latter further divided into the following chronological and bone age groups: 10-11.99, 12-13.99, 14-16 yr. In these subjects we evaluated auxological-anthropometric and biological maturity characteristics and endocrine parameters (cortisol, dehydroepiandrosterone sulphate, testosterone). No significant difference was found between prepubertal athletes and controls concerning anthropometric and biological maturation parameters, whereas testosterone basal levels were significantly lower (p less than 0.05) and DHEAS values were significantly higher (p less than 0.05). Pubertal football players were significantly taller than controls (particularly at 14-16 yr chronological age), with a greater biacromial diameter after 12 yr chronological and bone age and thinner skinfolds at 12-13.99 chronological and bone age. They were more advanced in all biological maturation parameters i.e. pubic hair, testicular volume and bone age, particularly those subjects playing football for the greatest number of years and training time. In pubertal football players the increase in DHEAS (p less than 0.05) already seen in prepubertals is also combined with a significant increase in testosterone (p less than 0.0001) and cortisol (p less than 0.05). Thus football players DHEAS is already higher during prepuberty and this increase chronologically precedes the advance in all the auxological-maturative parameters typical of our pubertal subjects.(ABSTRACT TRUNCATED AT 250 WORDS)

Demostracion de antigenos en productos biologicos por coaglutinacion de estafilococos que contienen proteina A. / Demonstration of antigens in biological products by coagglutination of Staphylococcus containing vitamin A

La cepa Cowan I de Staphylococcus aureus contiene proteina A que una la bacteria a la porcion distal (Fc) de las IgG y deja libres los sitios de combinacion (Fab.).Al utilizar diferentes antisueros para cubrir de anticuerpos a S. aureus, se diapone de una tecnica para reconocer diferentes antigenos en liquidos biologicos. La coaglutinacao de los estafilococos sobre um portaobjetos es una prueba que una sensibilidad similar a al contrainmunoelectroforesis y ademas, resulta un procedimiento mas rapido y sencillo. En el Laboratorio de Inmunoquimica, la coagulacion se aplica a muestras de orina de ninos con fiebre tifoidea u absceso hepatico amibiano, utilizando bacterias a las cuales se unen los anticuerpos anti-Salmonella typhi y anti-Entamoeba histolytica. Se comenta que el uso de otros anticuerpos puede ampliar la utilidad de esta prueba como ayuda diagnostica en diferentes procesos patologicos y se menciona algunas posibles causas de reacciones falsas positivas y las precauciones que es necesario tomar para no confundir los resultados